Evaluation of the late cellular response to the use of autogenous staphylococcal vaccines.

Abstract

Context: The best known and potent of the ancient adjuvants is Freund's complete adjuvant, and in its composition includes dead bacteria, which are used to enhance antibody production. When the antigen is mixed with Freund's complete adjuvant and injected subcutaneously or intradermally, it is possible to obtain a remarkable potentiation of antibody production, especially with protein antigens. Objective: to evaluate the late cellular response after the use of autogenous staphylococcal bacterial vaccines, from January 2010 to December 2011. Procedures: it was an observational, longitudinal, prospective and quasi-experimental study, with the non-probabilistic sample by criteria, which was constituted by individuals older than 18 years prior informed consent to participate in the study, concluding with 87 patients. Exclusion, inclusion and exit criteria were taken into consideration. A work table was created where it was previously discussed in a coordinated manner, to proceed to carry out during the study, reaching consensus on the order of information compilation. Each patient was given staphylococcal vaccines according to the isolate in the culture medium; and applied autogenously. They are grown separately and are killed by purification and standardization through the McFarland opalescence method. The vaccination scheme was subcutaneous. Conclusions: the patients achieved a good late cellular response with autogenous staphylococcal vaccines after treatment, and that there was no relationship between the late cellular response and the different age groups, nor with the time of disease evolution.